ORIGINAL PAPER
Structural identification and in vitro antioxidant activities of anthocyanins in black chokeberry (Aronia melanocarpa Elliot)
1
College of Food Science and Technology, Hebei Normal University of Science and Technology, China
2
Hebei Key Laboratory of Natural Products Activity Components and Function, Hebei Normal University of Science and Technology,
Qinhuangdao, 066004, P.R. of China
3
Department of Food Science and Human Nutrition, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA
Submission date: 2021-10-29
Final revision date: 2021-11-06
Acceptance date: 2021-11-09
Online publication date: 2021-12-13
Publication date: 2021-12-20
Corresponding author
Bin Du
Hebei Key Laboratory of Natural Products Activity Components and Function, Hebei Normal University of Science and Technology, China
Hebei Key Laboratory of Natural Products Activity Components and Function, Hebei Normal University of Science and Technology, China
eFood 2021;2(4):201–208
KEYWORDS
antioxidant activityAronia melanocarpaisolationanthocyaninsstructure identificationliquid chromatography-mass spectrometry (LC-MS)
TOPICS
ABSTRACT
Anthocyanins is a natural edible pigment with many health benefits. The aim of this work was the identification of anthocyanins present in Aronia melanocarpa using mass spectrometric features. The anthocyanins of the A. melanocarpa were analyzed by UV-Vis, HPLC-DAD and LC-EIS/MS methods. The four important anthocyanins were identified as follows: cyanidin-3-galactoside (68.68%), cyanidin-3-arabinoside (25.62%), cyanidin-3-glucoside (5.28%) and cyanidin-3-xyloside (0.42%). Among the four anthocyanin monomers, three anthocyanins with the highest content of A. melanocarpa were selected, and the antioxidant activity was studied with the total anthocyanins. The antioxidant capacity was cyanidin-3-galactoside > total anthocyanin > cyanidin-3-arabinoside > cyanidin-3-glucoside. The activity of the four anthocyanin samples was greater than ascorbic acid. The methodology described in this study will provide an effective tool for anthocyanins identification. Our results suggested that anthocyanins from A. melanocarpa exhibited effective antioxidant activity. These findings may be crucial in future research concerning chokeberry based functional food products.
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